Investigation of the Gas Phase Cluster Formation of Amino Acids by High-Resolution Mass Spectrometry
Disciplines
Analytical Chemistry | Biochemistry
Abstract (300 words maximum)
Mass spectrometry emerges as an essential technique to investigate the non-covalent cluster formation among various small molecules such as amino acids and nucleosides. Amino acids receive specific attention as they represent the building blocks of protein and protein complexes and also the early step in the origin of life. The interactions among amino acids, including hydrogen bonding, electrostatic and hydrophobic, play a crucial role in shaping the structures and functions of biological molecules. This study focuses on investigating the clustering behavior of arginine, histidine, and serine employing electrospray ionization (ESI) coupled with high resolution Orbitrap Exploris 240 mass spectrometer. For arginine, our mass spectrometry experiment detected several homo clusters from dimer to 11-mer. The most stable cluster observed was noticed for tetramer at m/z 697.5. The 11-mer cluster was detected at m/z 1917.23. For histidine, several homo clusters of histidine from dimer to 17-mer are noticed. The most stable cluster observed was noticed for tetramer at m/z 1086.49 m/z. The 17-mer cluster was detected at m/z 2638.18. Lastly for serine, our mass spectrometry experiment obtained several homo clusters of serine from mono dimer to 13-mer. The most stable cluster observed was noticed for tetramer at m/z 1039.18. The 13-mer was detected at m/z 1221.15. Overall, the non-covalent gas phase cluster creation by basic and polar of amino acids reveals a very diverse pattern.
Academic department under which the project should be listed
CSM - Chemistry and Biochemistry
Primary Investigator (PI) Name
Mohammad A. Halim
Investigation of the Gas Phase Cluster Formation of Amino Acids by High-Resolution Mass Spectrometry
Mass spectrometry emerges as an essential technique to investigate the non-covalent cluster formation among various small molecules such as amino acids and nucleosides. Amino acids receive specific attention as they represent the building blocks of protein and protein complexes and also the early step in the origin of life. The interactions among amino acids, including hydrogen bonding, electrostatic and hydrophobic, play a crucial role in shaping the structures and functions of biological molecules. This study focuses on investigating the clustering behavior of arginine, histidine, and serine employing electrospray ionization (ESI) coupled with high resolution Orbitrap Exploris 240 mass spectrometer. For arginine, our mass spectrometry experiment detected several homo clusters from dimer to 11-mer. The most stable cluster observed was noticed for tetramer at m/z 697.5. The 11-mer cluster was detected at m/z 1917.23. For histidine, several homo clusters of histidine from dimer to 17-mer are noticed. The most stable cluster observed was noticed for tetramer at m/z 1086.49 m/z. The 17-mer cluster was detected at m/z 2638.18. Lastly for serine, our mass spectrometry experiment obtained several homo clusters of serine from mono dimer to 13-mer. The most stable cluster observed was noticed for tetramer at m/z 1039.18. The 13-mer was detected at m/z 1221.15. Overall, the non-covalent gas phase cluster creation by basic and polar of amino acids reveals a very diverse pattern.