Native Mass Spectrometry Investigation of Organophosphate Pesticide Interaction with Cytochrome C
Disciplines
Analytical Chemistry | Environmental Chemistry | Environmental Health and Protection | Environmental Monitoring
Abstract (300 words maximum)
Pesticides are a major component of global agriculture. They are used to protect crops from pests and to ensure consumers have produce to purchase in grocery stores, local or international. However, pesticides can heavily destroy environments that are applied with it such as trophic poisoning and destruction of micro-food chains and if not properly applied and cleaned, even consumers can be affected by long term low concentration exposure. This brings a major importance to study pesticides as they are developed to ensure the environment isn’t extensively harmed and that individuals don’t suffer consequences from prolonged low concentration exposure. In this study, we employed native mass spectrometry approach to investigate the interaction of organophosphate herbicides with a heme-containing model protein, Cytochrome C, a mitochondrial protein that is essential in cellular respiration. Various buffer and charge reducing agents such as ammonium bicarbonate, ammonium acetate, ammonium hydroxide, and imidazole were used for obtaining the native mass spectrometry spectra of Cytochrome C with glyphosate. In presence of ammonium hydroxide, ammonium acetate and imidazole, various charge states of Cytochrome C from +5 to +10 were noticed where 8+ was the most intense peak, however, in the presence of imidazole, 6+ was the most intense charge state. Nonetheless, only few glyphosates were abducted with the protein, where at most would adduct two or three glyphosates at their most intense charge state. In presence of 10% methanol, mass spectrum of cytochrome C shows the charge state distribution from 6+ to 11+ where 8+ is the most intense peak and displayed the highest quantity of glyphosate adduction of three from charge states 8+ and 7+. A series of glyphosate from one to three were adducted with protein despite having the partially folded or unfolded gas phase structures of cytochrome C.
Academic department under which the project should be listed
CSM - Chemistry and Biochemistry
Primary Investigator (PI) Name
Mohammad A. Halim
Native Mass Spectrometry Investigation of Organophosphate Pesticide Interaction with Cytochrome C
Pesticides are a major component of global agriculture. They are used to protect crops from pests and to ensure consumers have produce to purchase in grocery stores, local or international. However, pesticides can heavily destroy environments that are applied with it such as trophic poisoning and destruction of micro-food chains and if not properly applied and cleaned, even consumers can be affected by long term low concentration exposure. This brings a major importance to study pesticides as they are developed to ensure the environment isn’t extensively harmed and that individuals don’t suffer consequences from prolonged low concentration exposure. In this study, we employed native mass spectrometry approach to investigate the interaction of organophosphate herbicides with a heme-containing model protein, Cytochrome C, a mitochondrial protein that is essential in cellular respiration. Various buffer and charge reducing agents such as ammonium bicarbonate, ammonium acetate, ammonium hydroxide, and imidazole were used for obtaining the native mass spectrometry spectra of Cytochrome C with glyphosate. In presence of ammonium hydroxide, ammonium acetate and imidazole, various charge states of Cytochrome C from +5 to +10 were noticed where 8+ was the most intense peak, however, in the presence of imidazole, 6+ was the most intense charge state. Nonetheless, only few glyphosates were abducted with the protein, where at most would adduct two or three glyphosates at their most intense charge state. In presence of 10% methanol, mass spectrum of cytochrome C shows the charge state distribution from 6+ to 11+ where 8+ is the most intense peak and displayed the highest quantity of glyphosate adduction of three from charge states 8+ and 7+. A series of glyphosate from one to three were adducted with protein despite having the partially folded or unfolded gas phase structures of cytochrome C.