Menthol-Thymol Based Deep Eutectic Solvents and Its Application for Hydrophobic Drug Characterization by Liquid Chromatography
Disciplines
Analytical Chemistry | Environmental Chemistry
Abstract (300 words maximum)
For many years, organic solvents have been used for various applications. Solvents like these are carcinogenic, flammable, and toxic when humans and other animals are exposed. These solvents are not considered eco-friendly. Deep eutectic solvents or DESs aim to combat these drawbacks. DESs can be prepared with cheap, biodegradable, and safe ingredients. DES formation is possible due to the hydrogen bonds between its components. In addition, DES has a freezing point much lower than its components. In this research project, menthol and thymol were used to synthesize a DES. This was done by achieving a 1:1 mole ratio of thymol and menthol. The mixture was then heated for 30 minutes at a temperature of 60°C with stirring at 500 rpm. Attenuated Total Reflection (ATR) coupled FT-IR spectroscopy technique was used to confirm the DES synthesis. In the DES IR spectrum, OH stretching is detected at ~3250 cm 1, which involves hydrogen bonding. In addition, C-H stretching is noticed at 2900 cm-1. Solubility of hydrophobic drug, ibuprofen, in DES is investigated by the Agilent 1290 infinity II UHPLC. Various ibuprofen samples were prepared by adding 1%, 5%, 10%, and 20% of DES. The chromatographic peak shape is improved when ibuprofen samples were prepared with DES. For instance, when 10% DES was added to ibuprofen solution, the peak area and height are significantly changed to 4883 mAU·s and 1151 mAU, respectively, confirming that the chromatographic performance of ibuprofen is significantly enhanced.
Academic department under which the project should be listed
CSM - Chemistry and Biochemistry
Primary Investigator (PI) Name
Mohammad A. Halim
Menthol-Thymol Based Deep Eutectic Solvents and Its Application for Hydrophobic Drug Characterization by Liquid Chromatography
For many years, organic solvents have been used for various applications. Solvents like these are carcinogenic, flammable, and toxic when humans and other animals are exposed. These solvents are not considered eco-friendly. Deep eutectic solvents or DESs aim to combat these drawbacks. DESs can be prepared with cheap, biodegradable, and safe ingredients. DES formation is possible due to the hydrogen bonds between its components. In addition, DES has a freezing point much lower than its components. In this research project, menthol and thymol were used to synthesize a DES. This was done by achieving a 1:1 mole ratio of thymol and menthol. The mixture was then heated for 30 minutes at a temperature of 60°C with stirring at 500 rpm. Attenuated Total Reflection (ATR) coupled FT-IR spectroscopy technique was used to confirm the DES synthesis. In the DES IR spectrum, OH stretching is detected at ~3250 cm 1, which involves hydrogen bonding. In addition, C-H stretching is noticed at 2900 cm-1. Solubility of hydrophobic drug, ibuprofen, in DES is investigated by the Agilent 1290 infinity II UHPLC. Various ibuprofen samples were prepared by adding 1%, 5%, 10%, and 20% of DES. The chromatographic peak shape is improved when ibuprofen samples were prepared with DES. For instance, when 10% DES was added to ibuprofen solution, the peak area and height are significantly changed to 4883 mAU·s and 1151 mAU, respectively, confirming that the chromatographic performance of ibuprofen is significantly enhanced.