Disciplines
Biotechnology | Computational Biology | Genomics
Abstract (300 words maximum)
Postpartum depression (PPD) is a very common mental disorder that affects 1 out of every 7 mothers. There is no known treatment for this mental condition among lactating mothers. However, 919 syrup is a Chinese herbal medicine that has been shown to improve conditions for people who are suffering from PPD. Since the 919 syrup is taken orally there is an interaction between the gut microbiota and the syrup. This shows that changes to the intestinal flora could be caused by the 919 syrup. The primary objective of the study is to find species of bacteria in the intestinal flora that may cause PPD. The secondary objective of the study is to find any phages that could modulate microbiomes in the gut that could cause harmful problems. This study will use metagenomic sequencing data that was gathered from the European Nucleotide Archive (ENA) accession number of PRJEB44528 using EBI. This data includes the entire metagenome of mouse fecal samples. The sequencing reads in the metagenome will then be trimmed using Cutadapt. Afterward, taxonomic classification will be conducted using Kraken2 with a custom database. The data can then be used to find which bacteria species could cause detrimental effects such as postpartum depression.
Academic department under which the project should be listed
CSM - Molecular and Cellular Biology
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Primary Investigator (PI) Name
Tsai-Tien Tseng
Identifying Intestinal Bacteria in Postpartum Stressed Female Mice
Postpartum depression (PPD) is a very common mental disorder that affects 1 out of every 7 mothers. There is no known treatment for this mental condition among lactating mothers. However, 919 syrup is a Chinese herbal medicine that has been shown to improve conditions for people who are suffering from PPD. Since the 919 syrup is taken orally there is an interaction between the gut microbiota and the syrup. This shows that changes to the intestinal flora could be caused by the 919 syrup. The primary objective of the study is to find species of bacteria in the intestinal flora that may cause PPD. The secondary objective of the study is to find any phages that could modulate microbiomes in the gut that could cause harmful problems. This study will use metagenomic sequencing data that was gathered from the European Nucleotide Archive (ENA) accession number of PRJEB44528 using EBI. This data includes the entire metagenome of mouse fecal samples. The sequencing reads in the metagenome will then be trimmed using Cutadapt. Afterward, taxonomic classification will be conducted using Kraken2 with a custom database. The data can then be used to find which bacteria species could cause detrimental effects such as postpartum depression.