Disciplines
Cancer Biology | Cell Anatomy | Cell Biology | Laboratory and Basic Science Research
Abstract (300 words maximum)
Utilization of Bioinformatics and Immunocytochemistry to Examine Gap Junction Expression in Breast Cancers Cells.
Jasmine D. Carter1, Giovanni Reyes1, Abeeha Choudhary2 and Eric A. Albrecht1
Breast cancer is known for its diverse clinical classifications and expressing different levels of membrane proteins such as ion channels and gap junctions. This diversity allows more variations in cell polarization, which can lead to enhanced directional ion fluxes in certain breast cancer subtypes. We utilized the interactive web portal UALCAN to evaluate the gene expression data of gap junctions, ion exchange channels and cytoskeletal proteins in breast cancer tissues. Our data showed several gene targets (e.g., GJA1(Cx43),GJB2(Cx26) increased expression during tumor development compared to normal breast tissue. Immunocytochemistry protocols were developed to examine the spatial expression of GJB2(Cx26) in MCF10A (normal breast cells) and MCF7 (weakly metastatic) breast cancer cell lines under static conditions. Primary antibodies to GJB2(Cx26) were visualized using fluorescein conjugated anti-mouse IgG (H+L) secondary antibodies. Our data suggests that the use of genomic and proteomic expression data is an effective approach for identifying differential expression differences in normal and malignant tissues.
1Kennesaw State University, Department of Molecular and Cellular Biology, Kennesaw, GA 30144
2Kennesaw State University, Department of Psychological Science, Kennesaw, GA 30144
Academic department under which the project should be listed
CSM - Molecular and Cellular Biology
Primary Investigator (PI) Name
Dr. Eric A Albrecht
Additional Faculty
Dr. Yizeng Li, Dept of Mechanical Engineering, yli54@kennesaw.edu Dr. Craig Brasco, School of Art and Design, cbrasco@kennesaw.edu Dr. Glenn Young, Dept of Mathematics, gyoung19@kennesaw.edu
Included in
Cancer Biology Commons, Cell Anatomy Commons, Cell Biology Commons, Laboratory and Basic Science Research Commons
Utilization of Bioinformatics and Immunocytochemistry to Examine Gap Junction Expression in Breast Cancers Cells
Utilization of Bioinformatics and Immunocytochemistry to Examine Gap Junction Expression in Breast Cancers Cells.
Jasmine D. Carter1, Giovanni Reyes1, Abeeha Choudhary2 and Eric A. Albrecht1
Breast cancer is known for its diverse clinical classifications and expressing different levels of membrane proteins such as ion channels and gap junctions. This diversity allows more variations in cell polarization, which can lead to enhanced directional ion fluxes in certain breast cancer subtypes. We utilized the interactive web portal UALCAN to evaluate the gene expression data of gap junctions, ion exchange channels and cytoskeletal proteins in breast cancer tissues. Our data showed several gene targets (e.g., GJA1(Cx43),GJB2(Cx26) increased expression during tumor development compared to normal breast tissue. Immunocytochemistry protocols were developed to examine the spatial expression of GJB2(Cx26) in MCF10A (normal breast cells) and MCF7 (weakly metastatic) breast cancer cell lines under static conditions. Primary antibodies to GJB2(Cx26) were visualized using fluorescein conjugated anti-mouse IgG (H+L) secondary antibodies. Our data suggests that the use of genomic and proteomic expression data is an effective approach for identifying differential expression differences in normal and malignant tissues.
1Kennesaw State University, Department of Molecular and Cellular Biology, Kennesaw, GA 30144
2Kennesaw State University, Department of Psychological Science, Kennesaw, GA 30144