Wound Healing Analysis for Venom Induced Cell Injury Models
Abstract (300 words maximum)
Wound and regeneration is an important aspect of organismal physiology. This study examined the use of imaging software to assess wound formation by Crotalus atrox venom. Three opensource macros were employed to identify and measure lesion areas (µm2) in cellular monolayers and compare their accuracy to manual tracings of the same lesions. Manual tracing of three separate wound micrographs displayed an average lesion area of 34,024 ± 21,903 µm2. Using the same micrographs, the wound healing size tool (WHST) macros calculated an average lesion area of 34,286 ± 19,928 µm2. In contrast, the SA_NJ macros and Wh_NJ macros reported an average lesion areas of 128,692 ± 338,811 µm2 and 2,376 ± 67 µm2, respectably. This suggests WHST more accurately identified and calculated the wound lesions produced from Crotalus atrox venom.
Academic department under which the project should be listed
CSM - Molecular and Cellular Biology
Primary Investigator (PI) Name
Eric Albrecht
Wound Healing Analysis for Venom Induced Cell Injury Models
Wound and regeneration is an important aspect of organismal physiology. This study examined the use of imaging software to assess wound formation by Crotalus atrox venom. Three opensource macros were employed to identify and measure lesion areas (µm2) in cellular monolayers and compare their accuracy to manual tracings of the same lesions. Manual tracing of three separate wound micrographs displayed an average lesion area of 34,024 ± 21,903 µm2. Using the same micrographs, the wound healing size tool (WHST) macros calculated an average lesion area of 34,286 ± 19,928 µm2. In contrast, the SA_NJ macros and Wh_NJ macros reported an average lesion areas of 128,692 ± 338,811 µm2 and 2,376 ± 67 µm2, respectably. This suggests WHST more accurately identified and calculated the wound lesions produced from Crotalus atrox venom.