Deep Eutectic Solvents Based Charge Reducing Agents for Native Mass Spectrometry
Disciplines
Analytical Chemistry | Biochemistry
Abstract (300 words maximum)
Electrospray ionization coupled with mass spectrometry (ESI-MS) has become a very powerful tool for protein analysis. However, formation of multiple charges sometimes leads to complex mass spectra due to overlapping of peaks and data interpretation becomes challenging, especially for large protein systems. A relatively new approach known as native mass spectrometry which also utilized electrospray ionization techniques obtaining the mass spectrum of large protein employing non-denaturing solvents. However, non-denaturing solvents only slightly reduce the charge states. To simplify the mass spectrum of a large protein system, various charge reducing agents such as triethylamine, trimethylamine oxide, imidazole, and alkali metal salts are used. In this study, we have investigated the charge reduction properties of amino acid based deep eutectic solvents (DESs). DESs solvents are greener, biodegradable, nonhazardous, cost effective and can be a better alternative to traditional charge reducing agents. DES is prepared by mixing two or more components in a certain ratio, one component acts as a hydrogen bond acceptor (HBA) and the other is a hydrogen bond donor (HBD). Several amino acids (Histidine, Lysine, Arginine, Proline and Serine) based on DESs were synthesized with glycerol. The DES formation was confirmed by the attenuated total reflection (ATR) coupled to IR spectroscopy and principal component analysis (PCA). Various concentrations of the DESs were mixed with a model protein, Lysozyme. When lysozyme was prepared in water, various charge states from 7+ to 11+ were noticed with the most dominant peak at 9+. For histidine-glycerol DES, the most dominant peak was spotted at 7+ with several change states ranging from 5+ to 8+. A significant charge reduction was observed for arginine-glycerol DES where the most abundant peak was detected at 5+ charge state. Similar trend is noticed for proline-glycerol DES. However, serine-glycerol DES failed to reduce the charge states.
Academic department under which the project should be listed
CSM - Chemistry and Biochemistry
Primary Investigator (PI) Name
Mohammad A. Halim
Deep Eutectic Solvents Based Charge Reducing Agents for Native Mass Spectrometry
Electrospray ionization coupled with mass spectrometry (ESI-MS) has become a very powerful tool for protein analysis. However, formation of multiple charges sometimes leads to complex mass spectra due to overlapping of peaks and data interpretation becomes challenging, especially for large protein systems. A relatively new approach known as native mass spectrometry which also utilized electrospray ionization techniques obtaining the mass spectrum of large protein employing non-denaturing solvents. However, non-denaturing solvents only slightly reduce the charge states. To simplify the mass spectrum of a large protein system, various charge reducing agents such as triethylamine, trimethylamine oxide, imidazole, and alkali metal salts are used. In this study, we have investigated the charge reduction properties of amino acid based deep eutectic solvents (DESs). DESs solvents are greener, biodegradable, nonhazardous, cost effective and can be a better alternative to traditional charge reducing agents. DES is prepared by mixing two or more components in a certain ratio, one component acts as a hydrogen bond acceptor (HBA) and the other is a hydrogen bond donor (HBD). Several amino acids (Histidine, Lysine, Arginine, Proline and Serine) based on DESs were synthesized with glycerol. The DES formation was confirmed by the attenuated total reflection (ATR) coupled to IR spectroscopy and principal component analysis (PCA). Various concentrations of the DESs were mixed with a model protein, Lysozyme. When lysozyme was prepared in water, various charge states from 7+ to 11+ were noticed with the most dominant peak at 9+. For histidine-glycerol DES, the most dominant peak was spotted at 7+ with several change states ranging from 5+ to 8+. A significant charge reduction was observed for arginine-glycerol DES where the most abundant peak was detected at 5+ charge state. Similar trend is noticed for proline-glycerol DES. However, serine-glycerol DES failed to reduce the charge states.