Disciplines
Biomedical Engineering and Bioengineering | Biotechnology | Mechanical Engineering | Medicine and Health Sciences
Abstract (300 words maximum)
Construction and design of giant unilamellar vesicles
Abstract
Giant Unilamellar Vesicles (GUV’s) consist of a phospholipid membrane bilayer and an aqueous space in between the membrane. Vesicles play a fundamental role in many fields of science, this is because you can incorporate different chemical compounds and proteins into the aqueous space of the vesicle. Thus, allowing for easier transport with less risk of negative exposure from harmful substances (Moscho et al., 1996). GUV’s are a simple model membrane that helps us study the divergent functions of more complex biological membranes (Protein Reconstitution Inside Giant Unilamellar Vesicles, 2022). In order to predict the outcome of actual interactions between GUV’s and other biological membranes. Multiple methods have since been established to configure both unilamellar and multilamellar vesicles. Some of which include ethanol-injection, hand-shake method (also known as thin-film), and emulsification method (Šturm and Poklar Ulrih, 2021). From previous research, and with the help of SolidWorks 2021 SP2.0 we know that the hand-shaking method would most likely yield the highest amount of unilamellar vesicles. Although all methods yield both multilamellar and unilamellar vesicles, the hand-shake method is the easiest to separate multilamellar vesicles into unilamellar vesicles. Thus, allowing for an easier experimental model to work with.
References
(1) MOSCHO, A., ORWAR, O., CHIU, D., MODI, B. and ZARE, R., 1996. Rapid preparation of Giant Unilamellar Vesicles. [online] Pnas.org. Available at:
(2) Annual Reviews. 2022. Protein Reconstitution Inside Giant Unilamellar Vesicles. [online] Available at:
(3) Šturm, L. and Poklar Ulrih, N., 2021. Basic Methods for Preparation of Liposomes and Studying Their Interactions with Different Compounds, with the Emphasis on Polyphenols. [online] Available at: [Accessed 20 March 2022].
Academic department under which the project should be listed
SPCEET - Mechanical Engineering
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Primary Investigator (PI) Name
Yizeng Li
Construction and Design of giant Unilamellar vesicles
Construction and design of giant unilamellar vesicles
Abstract
Giant Unilamellar Vesicles (GUV’s) consist of a phospholipid membrane bilayer and an aqueous space in between the membrane. Vesicles play a fundamental role in many fields of science, this is because you can incorporate different chemical compounds and proteins into the aqueous space of the vesicle. Thus, allowing for easier transport with less risk of negative exposure from harmful substances (Moscho et al., 1996). GUV’s are a simple model membrane that helps us study the divergent functions of more complex biological membranes (Protein Reconstitution Inside Giant Unilamellar Vesicles, 2022). In order to predict the outcome of actual interactions between GUV’s and other biological membranes. Multiple methods have since been established to configure both unilamellar and multilamellar vesicles. Some of which include ethanol-injection, hand-shake method (also known as thin-film), and emulsification method (Šturm and Poklar Ulrih, 2021). From previous research, and with the help of SolidWorks 2021 SP2.0 we know that the hand-shaking method would most likely yield the highest amount of unilamellar vesicles. Although all methods yield both multilamellar and unilamellar vesicles, the hand-shake method is the easiest to separate multilamellar vesicles into unilamellar vesicles. Thus, allowing for an easier experimental model to work with.
References
(1) MOSCHO, A., ORWAR, O., CHIU, D., MODI, B. and ZARE, R., 1996. Rapid preparation of Giant Unilamellar Vesicles. [online] Pnas.org. Available at:
(2) Annual Reviews. 2022. Protein Reconstitution Inside Giant Unilamellar Vesicles. [online] Available at:
(3) Šturm, L. and Poklar Ulrih, N., 2021. Basic Methods for Preparation of Liposomes and Studying Their Interactions with Different Compounds, with the Emphasis on Polyphenols. [online] Available at: [Accessed 20 March 2022].