Identification of a candidate akirin enhancer sequence
Disciplines
Cell and Developmental Biology | Genetics
Abstract (300 words maximum)
Akirin, a small nuclear protein with conserved function across eukaryotes, is a critical determinant in the development of functional, robust cardiac and skeletal patterning and musculature. Akirin serves as a transcription cofactor by acting as a link between transcription factors such as the gene Twist. Akirin uses chromatin remodeling complexes to ensure that Twist functions appropriately during transcription. If Akirin function is impaired, the resulting muscle patterning and structure is greatly impacted. We have identified a short sequence within the first intron of akirin that is highly conserved among closely related Drosophilid species. We are evaluating this sequence for possible promoter or enhancer activity. This evaluation is accomplished utilizing a variety of in vivo and in vitro techniques, both in live Drosophila embryos, as well as in cultured S2 cells. We have determined that a likely candidate enhancer sequence does indeed occur within this conserved element and are investigating a number of candidates that regulate this particular DNA sequence for akirin expression.
Academic department under which the project should be listed
CSM - Molecular and Cellular Biology
Primary Investigator (PI) Name
Scott Nowak
Identification of a candidate akirin enhancer sequence
Akirin, a small nuclear protein with conserved function across eukaryotes, is a critical determinant in the development of functional, robust cardiac and skeletal patterning and musculature. Akirin serves as a transcription cofactor by acting as a link between transcription factors such as the gene Twist. Akirin uses chromatin remodeling complexes to ensure that Twist functions appropriately during transcription. If Akirin function is impaired, the resulting muscle patterning and structure is greatly impacted. We have identified a short sequence within the first intron of akirin that is highly conserved among closely related Drosophilid species. We are evaluating this sequence for possible promoter or enhancer activity. This evaluation is accomplished utilizing a variety of in vivo and in vitro techniques, both in live Drosophila embryos, as well as in cultured S2 cells. We have determined that a likely candidate enhancer sequence does indeed occur within this conserved element and are investigating a number of candidates that regulate this particular DNA sequence for akirin expression.