Mass Spectrometry Based Untargeted Lipidomics Analysis of Shellfish
Disciplines
Analytical Chemistry | Aquaculture and Fisheries | Biochemistry | Food Chemistry
Abstract (300 words maximum)
Advanced mass spectrometry and powerful data handling system have led to discovery of all detectable lipids in a sample including unknows. Although classical targeted lipidomics approach focuses for accurate detection and quantification of very selected lipids, the goal of the untargeted lipidomics is to find increased coverage of lipids, often leading to discover new lipids. From this data, a greater understanding of why lipids are present in certain organisms can be used to track the lack, excess, or complexing of expected lipids. Shellfish have been widely known as a source of natural and essential lipids. The aim of this work is to detect and identify lipids present in various shellfish samples including lobster, crab, shrimp, and oysters. The samples were prepared using a floch extraction method, combining the desired sample with chloroform and methanol. The sample is placed on a shaker, incubated with water, and centrifuged. This creates a bottom chloroform layer that is collected, vacuum dried and resuspended with 9:1 methanol and toluene. The extracted samples are then placed in a well-plate in the sampler and lipids are separated by reverse phase liquid chromatography using C18 column in Vanquish Flex HPLC with a 12 min gradient. An Orbitrap Exploris 240 Mass Spectrometer was used to identify the lipids. Compound Discovery Software was used to search the LC-MS/MS data against the lipid database. After removing duplicates from the raw data and considering the named, there were 99 lipids in crab, 118 in lobster, 515 in shrimp, and 605 in oyster detected. Unsaturated fatty acids were the most prevalent lipid groups with fatty amides, sphingolipids, and fatty alcohols present in smaller percentages. Unsaturated fatty acids are crucial to many aspects of human health, including cardiovascular stability.
Academic department under which the project should be listed
CSM - Chemistry and Biochemistry
Primary Investigator (PI) Name
Mohammad A. Halim
Mass Spectrometry Based Untargeted Lipidomics Analysis of Shellfish
Advanced mass spectrometry and powerful data handling system have led to discovery of all detectable lipids in a sample including unknows. Although classical targeted lipidomics approach focuses for accurate detection and quantification of very selected lipids, the goal of the untargeted lipidomics is to find increased coverage of lipids, often leading to discover new lipids. From this data, a greater understanding of why lipids are present in certain organisms can be used to track the lack, excess, or complexing of expected lipids. Shellfish have been widely known as a source of natural and essential lipids. The aim of this work is to detect and identify lipids present in various shellfish samples including lobster, crab, shrimp, and oysters. The samples were prepared using a floch extraction method, combining the desired sample with chloroform and methanol. The sample is placed on a shaker, incubated with water, and centrifuged. This creates a bottom chloroform layer that is collected, vacuum dried and resuspended with 9:1 methanol and toluene. The extracted samples are then placed in a well-plate in the sampler and lipids are separated by reverse phase liquid chromatography using C18 column in Vanquish Flex HPLC with a 12 min gradient. An Orbitrap Exploris 240 Mass Spectrometer was used to identify the lipids. Compound Discovery Software was used to search the LC-MS/MS data against the lipid database. After removing duplicates from the raw data and considering the named, there were 99 lipids in crab, 118 in lobster, 515 in shrimp, and 605 in oyster detected. Unsaturated fatty acids were the most prevalent lipid groups with fatty amides, sphingolipids, and fatty alcohols present in smaller percentages. Unsaturated fatty acids are crucial to many aspects of human health, including cardiovascular stability.