Untargeted Animal and Plant Milk Lipidomics using Liquid Chromatography-Mass spectrometry
Disciplines
Biochemistry | Dairy Science
Abstract (300 words maximum)
Dairy is a large source of lipids necessary for most mammalian organisms to grow exponentially and a cornerstone of the human diet. These lipids are necessary for energy storage, vitamin absorption, and even brain development. The lipids in animal milks such as bovine and goat are vital as the main source of energy for growth, however, plant-based milk consumption, as part of a healthy diet, is continuously increasing. The objective of this research is to perform a comparative lipidomics study on the bovine, goat, coconut and almond milks and elucidate relationships between the lipid profile among them. The lipids were extracted from the milk sample employing Folch extraction protocol and were separated and dectected using Vanquish Flex HPLC coupled with Orbitrap Exploris 240 Mass Spectrometer. LC-MS data were proceeded through LIPID MAPS and ChemSpider databases for comprehensive analysis. Our current hypothesis is that mammalian milk will contain more fatty lipids, like fatty aldehydes, hydroxy fatty acids and saturated fats, associated with development and growth, while milks made from nuts may experience a larger variety of lipids and a higher concentration of unsaturated fats. However, the data has shown that while mammalian milk did correlate with the hypothesis of containing more saturated fats and much bulkier lipids like sterols and isoprenoids, it also contains a very similar variety of unsaturated fats compared to plant milks. Goat milk’s unsaturated fats make up 21.6% of its lipid variety, which is very close to coconut milk’s 21.4% and almond milk’s 24%, contradicting what is established by convention of plant-based milks being healthier due to containing more unsaturated fats. Some data even implying coconut milk contains a higher variety of lipids, like containing branched fatty acids and carboxylic fatty acids while no other milk did, while at the same time contained the lowest number of total lipids present in the sample.
Academic department under which the project should be listed
CSM - Chemistry and Biochemistry
Primary Investigator (PI) Name
Mohammad A. Halim
Untargeted Animal and Plant Milk Lipidomics using Liquid Chromatography-Mass spectrometry
Dairy is a large source of lipids necessary for most mammalian organisms to grow exponentially and a cornerstone of the human diet. These lipids are necessary for energy storage, vitamin absorption, and even brain development. The lipids in animal milks such as bovine and goat are vital as the main source of energy for growth, however, plant-based milk consumption, as part of a healthy diet, is continuously increasing. The objective of this research is to perform a comparative lipidomics study on the bovine, goat, coconut and almond milks and elucidate relationships between the lipid profile among them. The lipids were extracted from the milk sample employing Folch extraction protocol and were separated and dectected using Vanquish Flex HPLC coupled with Orbitrap Exploris 240 Mass Spectrometer. LC-MS data were proceeded through LIPID MAPS and ChemSpider databases for comprehensive analysis. Our current hypothesis is that mammalian milk will contain more fatty lipids, like fatty aldehydes, hydroxy fatty acids and saturated fats, associated with development and growth, while milks made from nuts may experience a larger variety of lipids and a higher concentration of unsaturated fats. However, the data has shown that while mammalian milk did correlate with the hypothesis of containing more saturated fats and much bulkier lipids like sterols and isoprenoids, it also contains a very similar variety of unsaturated fats compared to plant milks. Goat milk’s unsaturated fats make up 21.6% of its lipid variety, which is very close to coconut milk’s 21.4% and almond milk’s 24%, contradicting what is established by convention of plant-based milks being healthier due to containing more unsaturated fats. Some data even implying coconut milk contains a higher variety of lipids, like containing branched fatty acids and carboxylic fatty acids while no other milk did, while at the same time contained the lowest number of total lipids present in the sample.