Myogenesis in Drosophila melanogaster: Dissection of Distinct Muscle Types for Molecular Analysis

Authors

Anton L. Bryantsev, Department of Molecular and Cellular Biology, Kennesaw State University, Kennesaw, GA, USA.
Lizzet Castillo, Department of Biology, University of New Mexico, Albuquerque, NM, USA.
Sandy T. Oas, Department of Biology, University of New Mexico, Albuquerque, NM, USA.
Maria B. Chechenova, Department of Molecular and Cellular Biology, Kennesaw State University, Kennesaw, GA, USA.
Tracy E. Dohn, Department of Molecular and Cellular Biology, Kennesaw State University, Kennesaw, GA, USA.
TyAnna L. Lovato, Department of Biology, University of New Mexico, Albuquerque, NM, USA. tyanna@unm.edu.

Department

Molecular and Cellular Biology

Document Type

Article

Publication Date

1-1-2019

Abstract

Drosophila is a useful model organism for studying the molecular signatures that define specific muscle types during myogenesis. It possesses significant genetic conservation with humans for muscle disease causing genes and a lack of redundancy that simplifies functional analysis. Traditional molecular methods can be utilized to understand muscle developmental processes such as Western blots, in situ hybridizations, RT-PCR and RNAseq, to name a few. However, one challenge for these molecular methods is the ability to dissect different muscle types. In this protocol we describe some useful techniques for extracting muscles from the pupal and adult stages of development using flight and jump muscles as an example.

Journal Title

Methods in molecular biology (Clifton, N.J.)

Volume

1889

First Page

267

Last Page

281

Digital Object Identifier (DOI)

10.1007/978-1-4939-8897-6_16